THYMIDINE TRIPHOSPHATE SYNTHESIS IN TETRAHYMENA
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منابع مشابه
Thymidine Triphosphate Synthesis in Tetrahymena
The amount of thymidine-H(3) converted to thymidine-H(3) monophosphate in 30 min formed the basis for assays of thymidine kinase in cell extracts from Tetrahymena pyriformis. The optimal concentration of adenosine triphosphate is lower than that required by other cell types. Thymidine triphosphate does not exercise any feedback control of the enzyme. Other deoxyprimidine nucleotides were tested...
متن کاملFeedback Inhibition of Thymidine Kinase by Thymidine Triphosphate.
That the regulation of cell division and deoxyribonucleic acid synthesis is subject to some kind of chemical control in higher organisms is widely accepted (2-5). Davidson (2) has suggested that deoxyribonucleic acid synthesis takes place at critical concentrations of the deoxyribonucleoside triphosphates and that these compounds are components of a homeostatic mechanism. There are now at least...
متن کاملH3-thymidine Derivative Pools in Relation to Macronuclear Dna Synthesis in Tetrahymena Pyriformis
The formation of a soluble H(3)-thymidine derivative pool has been examined in Tetrahymena pyriformis as a function of macronuclear DNA synthesis during the cell life cycle. An autoradiographic technique which allows the detection of water-soluble materials within a cell has shown that these cells do not take up and retain exogenous H(3)-thymidine during G1 or G2. Uptake of H(3)-thymidine is re...
متن کاملEffects of thymidine on deoxyribonucleoside triphosphate pools and deoxyribonucleic acid synthesis in Chinese hamster ovary cells.
Cultured Chinese hamster ovary cells were synchronized by isoleucine starvation. Thymidine was added either during Gl or during S-phase and deoxyribonucleotide pools and the rate of DNA synthesis were measured. Addition of 1 mu thymidine to Gl cells did not appreciably influence the entry of the cells into S-phase but inhibited the rate of DNA synthesis up to 90%. The pools of dTTP, dGTP, and d...
متن کاملMitochondrial Incorporation of Tritiated Thymidine in Tetrahymena Pyriformis
Cytoplasmic D N A is known in a number of cell types. Hoff-JCrgenson and Zeuthen (7), using a microbial bioassay method, reported the presence of cytoplasmic D N A in frog eggs. Later, Hoff-J~6rgenson (8) and Elson et al. (4) indicated that sea urchin eggs contained a D N A reserve in the cytoplasm. Using autoradiographs of whole cells, Scherbaum (21) described cytoplasmic labeling in Ha-thymid...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1966
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.31.2.295